Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11082] to Caveolin-3 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P, IHC-FoFr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free
    See all Caveolin-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11082] to Caveolin-3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IHC-FoFrmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse skeletal muscle tissue lysate; IHC-Fr: Mouse skeletal muscle; ICC/IF: Differentiated C2C12 (Mouse myoblast cell line)

  • General notes

    ab249773 is the carrier-free version of ab173575.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
    Immunohistochemistry analysis of frozen mouse skeletal muscle tissue labeling Caveolin-3  with ab173575 at 1/1000 dilution. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, AlexaFluor® 488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Membranous staining on mouse skeletal muscle, while negative staining on white adipose tissue.
     
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173575).
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)

    ab173575 at 1/50. C2C12 (Mouse myoblast cell line) cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an Alexa Fluor® 594-conjugated mouse anti-alpha tubulin antibody (1/200). Nuclei counterstained with DAPI (blue). 

    Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. No staining detected in undifferentiated C2C12 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173575).
  • Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
    Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
    All lanes : Anti-Caveolin-3 antibody [EPR11082] (ab173575) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle tissue lysate at 20 µg
    Lane 2 : Mouse white adipose tissue lysate at 40 µg
    Lane 3 : Mouse brown adipose tissue lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Mouse adipose tissue (PMID: 8663016).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173575).
  • Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
    Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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