Anti-Cathepsin V antibody [BV55-1] (ab24508)
![Anti-Cathepsin V antibody [BV55-1] (ab24508)](https://www.abcam.com/ps/products/24/ab24508/Images/ab24508-35242-anti-cathepsin-v-antibody-bv55-1-immunohistochemistry.jpg "Anti-Cathepsin V antibody [BV55-1] (ab24508)")
Key features and details
- Mouse monoclonal [BV55-1] to Cathepsin V
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cathepsin V antibody [BV55-1]
See all Cathepsin V primary antibodies -
Description
Mouse monoclonal [BV55-1] to Cathepsin V -
Host species
Mouse -
Specificity
This antibody recognizes human Cathepsin L2 and Procathepsin L2, but not other Cathepsins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide surrounding amino acid 21 of human cathepsin L2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
BV55-1 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin V antibody [BV55-1] (ab24508)IHC image of ab24508 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24508, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
