HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Purified protein, Serum, Tissue Lysate, Urine
- Sensitivity: 50 ng
Overview
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Product name
HRV 3C Protease Activity Assay Kit (Colorimetric)
See all Human Rhinovirus 3C protease kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Purified protein, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
50 ng -
Product overview
HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088) provides a convenient method for detecting HRV 3C Protease activity in mammalian tissue samples, biological fluids and from purified proteins. The assay kit is based on the ability of a 3C Protease (derived from a HRV rhinovirus-14) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity from as low as 50 ng protein present in samples.
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Notes
Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes.
Human rhinovirus 3C protease (EC: 3.4.22.28) is a cysteine protease that recognizes the cleavage site Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 100 tests HRV 3C Protease Assay Buffer 1 x 25ml pNA Standard (0.1 M) 1 x 20µl HRV 3C Protease (Positive Control) 1 x 10µl HRV 3C Protease Substrate 1 x 500µl -
Research areas
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Relevance
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
Images
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HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)
Typical pNA standard calibration curve.
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HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)Kinetic progress curves at OD 405 nm for different amounts of HRV 3C Protease (positive control) after 1 hour incubation. Assays were performed following the kit protocol.
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HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)Change in OD 405 nm for different amounts of HRV 3C Protease(positive control) calculated from kinetic curves (previous figure).