Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10723(B)] to Cathepsin V
- Suitable for: WB, ICC/IF, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Cathepsin V antibody [EPR10723(B)]
See all Cathepsin V primary antibodies -
Description
Rabbit monoclonal [EPR10723(B)] to Cathepsin V -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide corresponding to residues in Human Cathepsin V (UniProt ID: O60911).
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Positive control
- A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10723(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution
Lane 1 : A549 lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : fetal kidney lysate
Lane 5 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
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Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
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Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
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Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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