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Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20921-83] to Caspase-4 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free
    See all Caspase-4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20921-83] to Caspase-4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1, IM-9 and KARPAS-299 whole cell lysate. Flow Cyt: THP-1 and IM-9 cells.

  • General notes

    Ab256303 is the carrier-free version of ab238124. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256303 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)
    Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized IM-9 (human multiple myeloma B Lymphoblast) cell line labeling Caspase 4 with ab238124 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238124).
  • Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)
    Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)

    Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling Caspase-4 with ab238124 at 1/60 (right panel, red) compared with HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (left panel, red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Negative control: HEK-293T (PMID: 25119034).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238124).
  • Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)
    Anti-Caspase-4 antibody [EPR20921-83] - BSA and Azide free (ab256303)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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