Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20921-83] to Caspase-4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-4 antibody [EPR20921-83]
    See all Caspase-4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20921-83] to Caspase-4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1, IM-9 and KARPAS-299 whole cell lysate. Flow Cyt: THP-1 and IM-9 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
    Western blot - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
    All lanes : Anti-Caspase-4 antibody [EPR20921-83] (ab238124) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 2 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
    Lane 3 : IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 32,38,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Negative control: HEK-293T and Jurkat (PMID: 25119034). 

    The molecular weight observed is consistent with the literatures (PMID: 22246630, PMID: 25119034, PMID: 24879791, PMID: 15123740).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
    Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

    Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling Caspase-4 with ab238124 at 1/60 (right panel, red) compared with HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (left panel, red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Negative control: HEK-293T (PMID: 25119034).

  • Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
    Flow Cytometry - Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized IM-9 (human multiple myeloma B Lymphoblast) cell line labeling Caspase 4 with ab238124 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody.

  • Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
    Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Caspase-4 antibody [EPR20921-83] (ab238124)

© 2021 Astana Biomed Group LLP. All rights reserved.