Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y154] to Caspase-2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-2 antibody [Y154] - BSA and Azide free
    See all Caspase-2 primary antibodies

  • Description

    Rabbit monoclonal [Y154] to Caspase-2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247211 is the carrier-free version of ab32021 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
    Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)

    This data was developed using ab32021, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
    Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
    Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat cell lysate

    Predicted band size: 49 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 18 kDa (possible cleavage fragment)



    This data was developed using ab32021, the same antibody clone in a different buffer formulation.

     

  • Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
    Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)

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