Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
![Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)](https://www.abcam.com/ps/products/247/ab247211/Images/ab247211-403201-anti-caspase-2-antibody-y154-bsa-and-azide-free-western-blot.jpg "Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y154] to Caspase-2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Caspase-2 antibody [Y154] - BSA and Azide free
See all Caspase-2 primary antibodies -
Description
Rabbit monoclonal [Y154] to Caspase-2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247211 is the carrier-free version of ab32021 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Y154 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
This data was developed using ab32021, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)](https://www.abcam.com/ps/products/247/ab247211/Images/ab247211-399255-anti-caspase-2-antibody-y154-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible cleavage fragment)This data was developed using ab32021, the same antibody clone in a different buffer formulation.
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![Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)](https://www.abcam.com/ps/products/247/ab247211/Images/ab247211-3-benefits-of-recombinant-antibodies.png)
Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
