Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM161] to Histone H3 (acetyl K9)
Suitable for: ICC/IF, WB, ELISA, ICC, ChIP
Reacts with: Human

Product name

Anti-Histone H3 (acetyl K9) antibody [RM161]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [RM161] to Histone H3 (acetyl K9)
Host species

Rabbit
Specificity

Reacts to Histone H3 (acetyl K9). No cross reactivity with other acetylated Lysines in histone H3.

Tested Applications & Species

Application	Species
ChIP	Human
ELISA	Human
ICC	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Histone H3 (N terminal) (acetyl K9).
Positive control
- WB: HeLa cells treated with sodium butyrate. ICC/IF: HeLa and HepG2 cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

RM161
Isotype

IgG
Research areas

ChIP - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

ChIP performed on HeLa cells using ab218553 at 5ug. Real-time PCR was performed using primers specific to the gene indicated.
Immunocytochemistry - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

Immunocytochemical analysis of HepG2 cells labeling Histone H3 (acetyl K9) using ab218553 at 1 µg/mL.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

Immunocytochemistry analysis of HeLa cells treated with sodium butyrate labeling Histone H3 (acetyl K9) using ab218553 at 2 μg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using ab218553 at 1 ug/mL as the capture antibody and biotinylated anti-H3K9ac as the detection antibody.
Western blot - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

All lanes : Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553) at 0.25 µg/ml

Lane 1 : HeLa cells untreated
Lane 2 : HeLa cells treated with sodium butyrate
ELISA - Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

ab218553 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Anti-Histone H3 (acetyl K9) antibody [RM161] (ab218553)

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