Caspase-1 Inhibitor Assay Kit (ab102489)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
Overview
-
Product name
Caspase-1 Inhibitor Assay Kit
See all Caspase-1 kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Assay time
1h 30m -
Product overview
Abcam's Caspase 1 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting. -
Notes
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-1 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate YVAD-AFC 1 x 0.5ml DTT 1 x 100µl -
Research areas
-
Relevance
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis. -
Cellular localization
Cytoplasmic -
Alternative names
- CASP-1
- CASP1
- CASP1 nirs variant 1
see all
Images
-
Caspase 1 Inhibitor Drug Detection Kit (ab102489)
Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using YVAD-AFC as caspase 1 substrate and following Caspase 1 Inhibitor Drug Detection Kit (ab102489) protocol. Lanes:
1.- Background Control: no Caspase 1, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 1, +z-VAD-FMK
3.- Positive Control: + Caspase 1, no z-VAD-FMK
4.- Caspase 1 + 2.5µM z-VAD-FMK
5.- Caspase 1 + 10µM z-VAD-FMK
6.- Caspase 1 + 40µM z-VAD-FMK
