Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free
    See all Cannabinoid Receptor I primary antibodies

  • Description

    Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICCmore details
    Unsuitable for: IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain and hippocampus tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate; SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate; NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. ICC: SH-SY5Y cells; mouse primary neuron; rat primary neuron. Flow Cyt: SH-SY5Y cells.

  • General notes

    ab278508 is the carrier-free version of ab259323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278508 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human hippocampus tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: Lane 1: 3 minutes; Lanes 2-4: 70 seconds.

  • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling Cannabinoid receptor 1 with ab259323 at 1/100 (5.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Flow Cytometry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    This data was developed using ab259323, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 67 seconds; Lane 2: 3 minutes.

  • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    This data was developed using ab259323, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 9655502 ).

    Negative control: mouse liver (PMID: 9655502).

    Exposure time: 70 seconds

  • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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