Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3536(2)] to CAMK1D - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free
    See all CAMK1D primary antibodies

  • Description

    Rabbit monoclonal [EPR3536(2)] to CAMK1D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249712 is the carrier-free version of ab172618.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)
    Western blot - Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)

    This data was developed using ab172618, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: CAMK1D knockout HAP1 cell lysate (20 µg) 

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)
    Western blot - Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)
    All lanes : Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution

    Lane 1 : Human fetal forebrain tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa



    This data was developed using ab172618, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)
    Immunocytochemistry - Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)
    This data was developed using ab172618, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).
  • Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)
    Anti-CAMK1D antibody [EPR3536(2)] - BSA and Azide free (ab249712)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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