Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4228] to Calsequestrin 2 - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free
See all Calsequestrin 2 primary antibodies
Description

Rabbit monoclonal [EPR4228] to Calsequestrin 2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab247652 is the carrier-free version of ab108381 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247652 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4228
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscles using unpurified ab108381 at a dilution of 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/10000 dilution (purified)

Lane 1 : Human fetal heart lysates
Lane 2 : Human skeletal muscle lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 46 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Calsequestrin 2 with Purified ab108381 at 1:250 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/1000 dilution (unpurified)

Lane 1 : Human heart lysate
Lane 2 : Human skeletal muscle lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

This data was developed using ab108381, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscles using unpurified ab108381 at a dilution of 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal breast tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal kidney tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal colon tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal tonsil tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com