Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4228] to Calsequestrin 2 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free
    See all Calsequestrin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4228] to Calsequestrin 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247652 is the carrier-free version of ab108381 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247652 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

    This data was developed using ab108381, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscles using unpurified ab108381 at a dilution of 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/10000 dilution (purified)

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human skeletal muscle lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108381, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

    This data was developed using ab108381, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Calsequestrin 2 with Purified ab108381 at 1:250 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/1000 dilution (unpurified)

    Lane 1 : Human heart lysate
    Lane 2 : Human skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using ab108381, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

    This data was developed using ab108381, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscles using unpurified ab108381 at a dilution of 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal brain tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal breast tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal kidney tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal colon tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    This data was developed using ab108381, the same antibody clone in a different buffer formulation.Unpurified ab108381 showing negative staining in Normal tonsil tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
    Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)

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