Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calmegin antibody [EPR11832-11] (ab172477)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11832-11] to Calmegin
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calmegin antibody [EPR11832-11]
    See all Calmegin primary antibodies

  • Description

    Rabbit monoclonal [EPR11832-11] to Calmegin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
    Database link: O14967

  • Positive control

    • Human testis, Mouse testis and Rat testis tissue lysate and Jurkat cell lysate; Human testis tissue; Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477) This image is courtesy of an abreview from Zachary Yu-Ching Lin

    IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

  • Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477)
    Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477)
    All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Rat testis tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (ab172477)
    Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.

  • Flow Cytometry - Anti-Calmegin antibody [EPR11832-11] (ab172477)
    Flow Cytometry - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).

  • Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477)
    Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477)

    Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.

  • Anti-Calmegin antibody [EPR11832-11] (ab172477)
    Anti-Calmegin antibody [EPR11832-11] (ab172477)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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