Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Calmegin antibody [EPR11832-11] (ab172477)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11832-11] to Calmegin
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Calmegin antibody [EPR11832-11]
See all Calmegin primary antibodies
Description

Rabbit monoclonal [EPR11832-11] to Calmegin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
Database link: O14967
Positive control
- Human testis, Mouse testis and Rat testis tissue lysate and Jurkat cell lysate; Human testis tissue; Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11832-11
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477) This image is courtesy of an abreview from Zachary Yu-Ching Lin

IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
See Abreview
Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477)

All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution

Lane 1 : Human testis tissue lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat testis tissue lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (ab172477)

Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.
Flow Cytometry - Anti-Calmegin antibody [EPR11832-11] (ab172477)

Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).
Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477)

Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.
Anti-Calmegin antibody [EPR11832-11] (ab172477)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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