IHC-P image of Calmegin staining with ab171971 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab171971 (1/500 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

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All lanes : Anti-Calmegin antibody [EPR11832] (ab171971) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Human testis lysate
Lane 3 : Mouse testis lysate
Lane 4 : Rat testis lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 70 kDa

Immunofluorescence analysis of Jurkat cells, labeling Calmegin using ab171971 at a 1/50 dilution.

Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling Calmegin using ab171971 at a 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

ab171971 showing -ve staining in Human normal brain tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

ab171971 showing -ve staining in Human skeletal muscle tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

ab171971 showing -ve staining in Human normal tonsil tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

ab171971 showing -ve staining in Human normal lung tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot analysis on immunoprecipitation pellet from Human testis lysate, labeling Calmegin using ab171971 at a 1/10 dilution and an IgG control.