Anti-Calmegin antibody - C-terminal (ab191556)
Key features and details
- Rabbit polyclonal to Calmegin - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-Calmegin antibody - C-terminal
See all Calmegin primary antibodies -
Description
Rabbit polyclonal to Calmegin - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
-
Immunogen
Synthetic peptide corresponding to Mouse Calmegin aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P52194 -
Positive control
- Mouse testis extract.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Calmegin antibody - C-terminal (ab191556)All lanes : Anti-Calmegin antibody - C-terminal (ab191556) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse sperm cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 69 kDa
-
Immunoprecipitation - Anti-Calmegin antibody - C-terminal (ab191556)Immunoprecipitation of Calmegin from mouse testis lysate (1 mg) using 2 μl of ab191556 and 50 μl of protein A conjugated magnetic beads. The immunoprecipitated sample was analyzed by western blotting with ab191556 at 1/1000 dilution.
1. Input 2. non immune serum 3. ab191556 -
Western blot - Anti-Calmegin antibody - C-terminal (ab191556)All lanes : Anti-Calmegin antibody - C-terminal (ab191556) at 1/1000 dilution
Lane 1 : Triton X 100 extracts from wild type mouse testis
Lane 2 : Triton X 100 extracts from Calmegin knockout mouse testis
Lysates/proteins at 20 µg per lane.
Predicted band size: 69 kDa
