Anti-C2 antibody (ab77498)
Key features and details
- Rabbit polyclonal to C2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C2 antibody
See all C2 primary antibodies -
Description
Rabbit polyclonal to C2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human C2 aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94384) -
Positive control
- This antibody gave a positive signal in Human Plasma Total Protein Preparation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications ChimpanzeeGorillaOrangutanApplication Abreviews Notes IHC-P Use a concentration of 1 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).Notes IHC-P
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).Target
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Function
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
Involvement in disease
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 717 Human
- Omim: 217000 Human
- SwissProt: P06681 Human
- Unigene: 408903 Human
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Alternative names
- ARMD14 antibody
- C2 antibody
- C3/C5 convertase antibody
see all
Images
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Western blot - Anti-C2 antibody (ab77498)Anti-C2 antibody (ab77498) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
C2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 105 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies this target. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2 antibody (ab77498)IHC image of Ab77498 staining in Human Liver Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab77498, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab77498 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-C2 antibody (ab77498)Anti-C2 antibody (ab77498) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
C2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 105 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies this target. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2 antibody (ab77498)IHC image of Ab77498 staining in Human Liver Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab77498, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
