Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)
![Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)](https://www.abcam.com/ps/products/251/ab251512/Images/ab251512-403423-anti-c2-antibody-epr17979-bsa-and-azide-free-western-blot.jpg "Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17979] to C2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-C2 antibody [EPR17979] - BSA and Azide free
See all C2 primary antibodies -
Description
Rabbit monoclonal [EPR17979] to C2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251512 is the carrier-free version of ab209900. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251512 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17979 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)All lanes : Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?This data was developed using ab209900, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1 and 3: 3 minutes; Lane 2: 30 seconds.
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![Western blot - Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)](https://www.abcam.com/ps/products/251/ab251512/Images/ab251512-399220-anti-c2-antibody-epr17979-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution + Mouse serum at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis data was developed using ab209900, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)](https://www.abcam.com/ps/products/251/ab251512/Images/ab251512-3-benefits-of-recombinant-antibodies.png)
Anti-C2 antibody [EPR17979] - BSA and Azide free (ab251512)
