Recombinant human MAP3K8/COT protein (Active) (ab271472)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human MAP3K8/COT protein (Active)
See all MAP3K8/COT proteins and peptides -
Biological activity
Specific activity = 47 pmol/min/µg.
100 µl of 240 nM biotinylated MEK1 peptide was coated onto a neutravidin plate for 1 hour at 37°C. After overnight blocking with TBS superblock, the reaction was performed with 10 µM ATP for 60 minutes at RT, and phospho-MEK1 was detected using an anti-phospho-MEK1 antibody. Relative specific activity was determined using a standard kinase under the same conditions.
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Purity
>= 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MENLYASEEP AVYEPSLMTM CQDSNQNDER SKSLLLSGQE VPWLSSVRYG TVEDLLAFAN HISNTAKHFY GQRPQESGIL LNMVITPQNG RYQIDSDVLL IPWKLTYRNI GSDFIPRGAF GKVYLAQDIK TKKRMACKLI PVDQFKPSDV EIQACFRHEN IAELYGAVLW GETVHLFMEA GEGGSVLEKL ESCGPMREFE IIWVTKHVLK GLDFLHSKKV IHHDIKPSNI VFMSTKAVLV DFGLSVQMTE DVYFPKDLRG TEIYMSPEVI LCRGHSTKAD IYSLGATLIH MQTGTPPWVK RYPRSAYPSY LYIIHKQAPP LEDIADDCSP GMRELIEASL ERNPNHRPRA ADLLKHEALN PPREDQPR -
Predicted molecular weight
72 kDa including tags -
Amino acids
30 to 397 -
Tags
His tag N-Terminus , GST tag N-Terminus -
Additional sequence information
Genbank: NM_005204
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Preparation and Storage
- AURA2
- c COT
- Cancer Osaka thyroid oncogene
Images
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Functional Studies - Recombinant Human MAP3K8/COT protein (Tagged) (ab271472)
Functional analysis of ab271472.
Specific activity = 47 pmol/min/µg.
100 µl of 240 nM biotinylated MEK1 peptide was coated onto a neutravidin plate for 1 hour at 37°C. After overnight blocking with TBS superblock, the reaction was performed with 10 µM ATP for 60 minutes at RT, and phospho-MEK1 was detected using an anti-phospho-MEK1 antibody. Relative specific activity was determined using a standard kinase under the same conditions.
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SDS-PAGE - Recombinant Human MAP3K8/COT protein (Tagged) (ab271472)SDS-PAGE analysis of ab271472.