Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15799] to C14orf166 - BSA and Azide free
  • Suitable for: ICC, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C14orf166 antibody [EPR15799] - BSA and Azide free
    See all C14orf166 primary antibodies

  • Description

    Rabbit monoclonal [EPR15799] to C14orf166 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250954 is the carrier-free version of ab188326.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    All lanes : Anti-C14orf166 antibody [EPR15799] (ab188326) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa



    This data was developed using ab188326, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    Immunocytochemistry - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    This data was developed using ab188326, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of U87-MG cells labeling C14orf166 using ab188326 at 1/100 dilution. A goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    This data was developed using ab188326, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    This data was developed using ab188326, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    This data was developed using ab188326, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of 293T cell lysate labeling C14orf166 using ab188326 at 1/50 dilution (Lane 1). An anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of 293T cell lysate.
  • Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    All lanes : Anti-C14orf166 antibody [EPR15799] (ab188326) at 1/10000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : 293T cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa



    This data was developed using ab188326, the same antibody clone in a different buffer formulation.

     

  • Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)
    Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (ab250954)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.