Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)
![Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)](https://www.abcam.com/ps/products/256/ab256492/Images/ab256492-355085-anti-c-reactive-protein-epr22862-10-western-blot-human.jpg "Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22862-10] to C Reactive Protein
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-C Reactive Protein antibody [EPR22862-10]
See all C Reactive Protein primary antibodies -
Description
Rabbit monoclonal [EPR22862-10] to C Reactive Protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human liver, Human plasma, Human kidney, Human lung cancer and Human colon cancer lysates. IHC-P: Human liver and Human liver cancer tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22862-10 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)All lanes : Anti-C Reactive Protein antibody [EPR22862-10] (ab256492) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human plasma lysate
Lane 3 : Human kidney tissue lysate
Lane 4 : Human lung cancer tissue lysate
Lane 5 : Human colon cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile is consistent with what has been described in the literature (PMID: 25812732, 28962140)
Exposure time: Lanes 1-2: 3 seconds Lanes 3-4: 59 seconds Lane 5: 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)](https://www.abcam.com/ps/products/256/ab256492/Images/ab256492-2-anti-c-reactive-protein-epr22862-10-immunohistochemistry-liver-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling C Reactive Protein with ab256492 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver cancer (PMID: 25812732, 30056637) is observed. The section was incubated with ab256492 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)](https://www.abcam.com/ps/products/256/ab256492/Images/ab256492-1-anti-c-reactive-protein-epr22862-10-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C Reactive Protein with ab256492 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver (PMID: 25812732, 30056637). The section was incubated with ab256492 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)](https://www.abcam.com/ps/products/256/ab256492/Images/ab256492-4-benefits-of-recombinant-antibodies.png)
Anti-C Reactive Protein antibody [EPR22862-10] (ab256492)
