Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C Reactive Protein antibody [Y284] (ab32412)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y284] to C Reactive Protein
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-C Reactive Protein antibody [Y284]
    See all C Reactive Protein primary antibodies

  • Description

    Rabbit monoclonal [Y284] to C Reactive Protein

  • Host species

    Rabbit

  • Specificity

    ab32412 recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2 as well as isoform 1.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human C Reactive Protein aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab216758)

  • Epitope

    ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.

  • Positive control

    • WB: Human serum and plasma lysates. IHC-P: Normal human liver tissue. IP: HepG2 whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
    Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
    All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)

    Lane 1 : Human serum
    Lane 2 : Human plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)
    Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)

    ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10µg)

    Lane 2 (+): ab32412 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
    Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
    Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum

    Predicted band size: 25 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.

  • Anti-C Reactive Protein antibody [Y284] (ab32412)
    Anti-C Reactive Protein antibody [Y284] (ab32412)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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