Human IL-34 ELISA Kit (ab235640)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.4 pg/ml
- Range: 28.1 pg/ml - 1800 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-34 ELISA Kit
See all IL-34 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Milk 9 8% Inter-assay Sample n Mean SD CV% Milk 3 11.6% -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.4 pg/ml -
Range
28.1 pg/ml - 1800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 106 104% - 110% Milk 113 111% - 115% Serum 84 81% - 88% Cell culture media 110 108% - 111% Hep Plasma 89 87% - 90% EDTA Plasma 92 89% - 96% Cit plasma 86 84% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-34 ELISA Kit (ab235640) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-34 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-34 with 3.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-34 Capture Antiibody 1 x 600µl 10X Human IL-34 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Human IL-34 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R. -
Tissue specificity
Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon. -
Sequence similarities
Belongs to the IL-34 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C16orf77
- Chromosome 16 open reading frame 77
- FPT025
see all -
Database links
- Entrez Gene: 146433 Human
- Omim: 612081 Human
- SwissProt: Q6ZMJ4 Human
- Unigene: 461214 Human
Images
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Other - Human IL-34 ELISA Kit (ab235640)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Competitor Curve ComparisonStandard curve comparison between human IL-34 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human IL-34 standard curve in Sample Diluent 50BP.The IL-34 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-34 in human milk samples.The concentrations of IL-34 were measured in duplicates, interpolated from the IL-34 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 18.75%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-34 concentration was determined to be 1,014 pg/mL in neat human milk.
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Sandwich ELISA - Human IL-34 ELISA Kit (ab235640)To learn more about the advantages of recombinant antibodies see here.
