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Anti-BTAF1 antibody [BTA3D61] (ab50990)

Key features and details

  • Mouse monoclonal [BTA3D61] to BTAF1
  • Suitable for: WB, IP, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-BTAF1 antibody [BTA3D61]
    See all BTAF1 primary antibodies

  • Description

    Mouse monoclonal [BTA3D61] to BTAF1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to BTAF1.

  • Positive control

    • WB: HEK293, NIH3T3 and F2408 whole cell lysates. ICC/IF and FACS: HeLa cells. IP: RIPA lysate of HeLa cells.

  • General notes

    Previously labelled as TAF172.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + HEK293 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 207 kDa
    Observed band size: 207 kDa

  • Immunocytochemistry - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Immunocytochemistry - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    HeLa cells stained with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200.
  • Immunoprecipitation - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Immunoprecipitation - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Ab50990 used at 5µg/0.5mg RIPA lysate of HeLa cells. The immunoprecipitate was subjected to Western blotting using ab50990 at 1/100. The ECL technique was used, and the secondary antibody was Mouse IgG at 1/2500.
  • Flow Cytometry - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Flow Cytometry - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Cells labelled with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/400 dilution.
  • Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 207 kDa
    Observed band size: 207 kDa

  • Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)
    Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 207 kDa
    Observed band size: 207 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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