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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIID

Anti-BTAF1 antibody (ab72285)

Anti-BTAF1 antibody (ab72285)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BTAF1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BTAF1 antibody
    See all BTAF1 primary antibodies
  • Description

    Rabbit polyclonal to BTAF1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human BTAF1 aa 75-125.
    Database link: NP_003963.1

  • Positive control

    • Whole cell lysates from HeLa and 293T cells.
  • General notes

    Previously labelled as TAF172.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIID
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Preinitiation complex

Images

  • Western blot - Anti-BTAF1 antibody (ab72285)
    Western blot - Anti-BTAF1 antibody (ab72285)
    All lanes : Anti-BTAF1 antibody (ab72285) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 207 kDa
    Observed band size: 207 kDa
    Additional bands at: 170 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation - Anti-BTAF1 antibody (ab72285)
    Immunoprecipitation - Anti-BTAF1 antibody (ab72285)

    Detection of Human BTAF1 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab72285 at 3µg/mg lysate for IP and at 1µg/ml for subsequent WB.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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