Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free (ab273157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23442-43] to BRCA2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free
    See all BRCA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23442-43] to BRCA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and MCF7 whole cell lysates.

  • General notes

    ab273157 is the carrier-free version of ab239375. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free (ab273157)
    Western blot - Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free (ab273157)
    All lanes : Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free (ab273157) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 40 µg (fresh lysate)
    Lane 2 : Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 40 µg (fresh lysate)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 384 kDa
    Observed band size: 384 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Capan-1 (PMID:16251802).

    Freshly prepared lysates are strongly recommended for WB detection. 

    Exposure time: 3 minutes.

    These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycerol. BSA and sodium azide (ab239375).

  • Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free (ab273157)
    Anti-BRCA2 antibody [EPR23442-43] - BSA and Azide free (ab273157)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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