Human on human IHC kit (HRP/DAB) (ab214749)
Overview
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Product name
Human on human IHC kit (HRP/DAB) -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human on human IHC kit (HRP/DAB) (ab214749) is designed for staining human primary antibodies on human tissues without background staining.
Antigen detection using a primary antibody of the same species as the test tissue yields high background when an indirect detection method is used. This severely limits the ability to screen human antibodies on human tissues. Abcam’s Human on human IHC kit (HRP/DAB) (ab214749) is a ready to use system that results in excellent sensitivity and high specificity using a simple protocol. Furthermore, as the protocol uses a polymer-based, biotin-free system, it does not require blocking of endogenous biotin.
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Tested applications
Suitable for: IHCmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 6 ml 18 ml 110 ml 20X DAB Chromogen 1 x 1.5ml 1 x 2ml 1 x 6ml 5X Quenching Buffer 1 x 1.5ml 2 x 2.3ml 2 x 13ml Blocking Buffer A 1 x 6ml 1 x 18ml 1 x 110ml Blocking Buffer B 1 x 6ml 1 x 18ml 1 x 110ml DAB Substrate Buffer 1 x 12ml 2 x 15ml 1 x 120ml Human HRP Polymer 1 x 6ml 1 x 18ml 1 x 110ml Human Primer 1 x 6ml 1 x 18ml 1 x 110ml
