Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13753-43] to BRAT1 - BSA and Azide free
  • Suitable for: IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free
    See all BRAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13753-43] to BRAT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250537 is the carrier-free version of ab181868 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250537 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BAAT1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)
    Western blot - Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)
    All lanes : Anti-BRAT1 antibody [EPR13753-43] - C-terminal (ab181868) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 88 kDa



    This data was developed using ab181868, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)
    Flow Cytometry - Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)
    This data was developed using ab181868, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling BRAT1 with ab181868 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Immunoprecipitation - Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)
    Immunoprecipitation - Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)
    This data was developed using ab181868, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181868 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)
    Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free (ab250537)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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