Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric) (ab273341)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
Overview
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Product name
Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric) (ab273341) is a simple plate-based fluorometric assay for measuring Hydroxy Acid Oxidase (HAO) activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H2O2. H2O2 then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction.
The assay can detect as low as 1 µU of HAO activity in biological samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests H2O2 Standard 1 x 100µl HAO Assay Buffer 1 x 25ml HAO Dilution Buffer 1 x 200µl HAO Enzyme Mix Lyophilized 1 vial HAO Positive Control 1 x 10µl HAO Probe 1 x 200µl HAO Substrate Lyophilized 1 vial
Images
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Example data
Example data.
H2O2 Standard Curve.
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Example dataExample data.
Enzyme kinetics of rat liver lysate (100 ng protein) and spinach leaf lysate (105 ng protein).
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Example dataExample data.
HAO specific activity in rat liver and spinach lysates.