Anti-beta III Tubulin antibody (ab41489)
Key features and details
- Chicken polyclonal to beta III Tubulin
- Suitable for: ICC/IF
- Isotype: IgY
Overview
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Product name
Anti-beta III Tubulin antibody
See all beta III Tubulin primary antibodies -
Description
Chicken polyclonal to beta III Tubulin -
Host species
Chicken -
Tested applications
Suitable for: ICC/IFmore details -
Immunogen
Three different synthetic peptides conjugated to KLH. These synthetic peptides corresponded to different regions of the beta III Tubulin gene product, but are shared between human and rat.
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General notes
Equal volumes of three different affinity-purified anti-peptide antibodies were combined to make this product.
Do not freeze antibody unless you want to store for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 99.98% 1X PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)
Immunocytochemistry/Immunofluorescence analysis of SpH21 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GAD67, Blue - DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)Immunocytochemistry/Immunofluorescence analysis of SpH64 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GFP, Blue: DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489) Image from Ohmine S et al., Stem Cell Research & Therapy 2011, 2:46, 16 November 2011. Fig 5.; doi: 10.1186/scrt87.Immunofluorescence analysis of HPC and PBMC blood-derived iPSC clones, staining beta III Tubulin (green) with ab41489.
Cells were fixed with 4% PFA and blocked for 30 minutes in PBS with 5% fetal bovine serum. Cells were incubated with primary antibody overnight at 4°C. A FITC-conjugated donkey anti-chicken IgG was used as the secondary antibody.
