Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)
![Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.com/ps/products/184/ab184167/Images/ab184167-393583-anti-peroxiredoxin-4-antibody-epr15458-western-blot-human-knockout-lysate.jpg "Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15458(B)] to Peroxiredoxin 4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Peroxiredoxin 4 antibody [EPR15458(B)]
See all Peroxiredoxin 4 primary antibodies -
Description
Rabbit monoclonal [EPR15458(B)] to Peroxiredoxin 4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, K562, HEK293T and Jurkat cell lysates. IHC-P: Human ovarian carcinoma tissue, Mouse liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15458(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)All lanes : Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PRDX4 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab184167 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.
ab184167 Anti-Peroxiredoxin 4 antibody [EPR15458(B)] was shown to specifically react with Peroxiredoxin 4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266727 (knockout cell lysate ab257264) was used. Wild-type and Peroxiredoxin 4 knockout samples were subjected to SDS-PAGE. ab184167 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.com/ps/products/184/ab184167/Images/ab184167-216571-anti-peroxiredoxin-4-antibody-epr15458b-immunohistochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Peroxiredoxin 4 with ab184167 at 1/250 dilution.
Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.com/ps/products/184/ab184167/Images/ab184167-216570-anti-peroxiredoxin-4-antibody-epr15458b-immunohistochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)Immunohistochemical analysis of, paraffin-embedded Human ovarian carcinoma tissue labeling Peroxiredoxin 4 with ab184167 at 1/250 dilution. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.com/ps/products/184/ab184167/Images/ab184167-216569-anti-peroxiredoxin-4-antibody-epr15458b-western-blot.png)
Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)All lanes : Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
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![Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.com/ps/products/184/ab184167/Images/ab184167-5-benefits-of-recombinant-antibodies.png)
Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)
