Anti-Peroxiredoxin 4 antibody (ab59542)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Peroxiredoxin 4 antibody
See all Peroxiredoxin 4 primary antibodies -
Description
Rabbit polyclonal to Peroxiredoxin 4 -
Host species
Rabbit -
Specificity
ab59542 reacts with Peroxiredoxin 4. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide corresponding to Human Peroxiredoxin 4 aa 65-83.
Sequence:SRVSVADHSLHLSKAKISK
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Peroxiredoxin 4 antibody (ab59542) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 74 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The band observed at 27 kDa could potentially be a cleaved form of Peroxiredoxin 4 due to the presence of a 39 amino acid signal peptide. -
ab59542 at 1/100 dilution staining Peroxiredoxin 4 in sebaceous glands in rat skin by Immunohistochemistry. Secondary antibody Jackson biotinylated Donkey anti-rabbit (1/2000) followed by Vector ABC kit and visualised with DAB substrate.
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ab59542 at 1/100 dilution staining Peroxiredoxin 4 in Non-ciliated bronchiolar cells (Clara cells) in rat lung by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).