Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta I Tubulin antibody [2A1A9] (ab204947)

Key features and details

  • Mouse monoclonal [2A1A9] to beta I Tubulin
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-beta I Tubulin antibody [2A1A9]
    See all beta I Tubulin primary antibodies

  • Description

    Mouse monoclonal [2A1A9] to beta I Tubulin

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human beta I Tubulin aa 1-200. expressed in E. Coli.
    Database link: Q9H4B7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Transfected HEK293 cell lysate; K562, HepG2, A431, Jurakat, Hela, NIH-3T3, Cos7, PC12 cell lysates; A431 cells, ovarian cancer tissues; Hela cells

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    2A1A9

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution + human beta 1 Tubulin (AA: 33-166) recombinant protein

    Predicted band size: 50 kDa

  • Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    All lanes : Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : human beta 1 Tubulin (AA: 33-166) -hIgGFc transfected HEK293 cell lysate

    Predicted band size: 50 kDa

  • Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    All lanes : Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Hela cell lysate
    Lane 6 : NIH-3T3 cell lysate
    Lane 7 : Cos7 cell lysate
    Lane 8 : PC12 cell lysate

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

    Immunofluorescent staining of Hela cells labeling beta I Tubulin with ab204947 at 1/200 (green).  Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow Cytometry - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Flow Cytometry - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

    Flow cytometric analysis of A431 cells labeling beta I Tubulin with ab204947 at 1/200 (green) or negative control (red)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

    Immunohistochemical analysis of formalin fixed paraffin embedded ovarian cancer tissue labeling beta I Tubulin using ab204947 at 1/200 dilution followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [2A1A9] (ab204947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

    Immunohistochemical analysis of formalin fixed paraffin embedded ovarian cancer tissue labeling beta I Tubulin using ab204947 at 1/200 dilution followed by DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-beta I Tubulin antibody [2A1A9] (ab204947)

© 2021 Astana Biomed Group LLP. All rights reserved.