Antibodies, Proteins, Kits and Reagents for Life Science

Anti-beta I Tubulin antibody [2A1A9] (ab204947)

Key features and details

Mouse monoclonal [2A1A9] to beta I Tubulin
Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
Reacts with: Mouse, Rat, Human, African green monkey
Isotype: IgG1

Product name

Anti-beta I Tubulin antibody [2A1A9]
See all beta I Tubulin primary antibodies
Description

Mouse monoclonal [2A1A9] to beta I Tubulin
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human beta I Tubulin aa 1-200. expressed in E. Coli.
Database link: Q9H4B7

Run BLAST with Run BLAST with
Positive control
- Transfected HEK293 cell lysate; K562, HepG2, A431, Jurakat, Hela, NIH-3T3, Cos7, PC12 cell lysates; A431 cells, ovarian cancer tissues; Hela cells

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

2A1A9
Isotype

IgG1
Research areas

Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution + human beta 1 Tubulin (AA: 33-166) recombinant protein

Predicted band size: 50 kDa
Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

All lanes : Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : human beta 1 Tubulin (AA: 33-166) -hIgGFc transfected HEK293 cell lysate

Predicted band size: 50 kDa
Western blot - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

All lanes : Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution

Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Hela cell lysate
Lane 6 : NIH-3T3 cell lysate
Lane 7 : Cos7 cell lysate
Lane 8 : PC12 cell lysate

Predicted band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

Immunofluorescent staining of Hela cells labeling beta I Tubulin with ab204947 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

Flow cytometric analysis of A431 cells labeling beta I Tubulin with ab204947 at 1/200 (green) or negative control (red)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

Immunohistochemical analysis of formalin fixed paraffin embedded ovarian cancer tissue labeling beta I Tubulin using ab204947 at 1/200 dilution followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [2A1A9] (ab204947)

Immunohistochemical analysis of formalin fixed paraffin embedded ovarian cancer tissue labeling beta I Tubulin using ab204947 at 1/200 dilution followed by DAB staining.

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