Human TUBB3 (beta III Tubulin) knockout HCT116 cell lysate (ab257070)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263502 - Human TUBB3 knockout HCT116 cell lysate 1 x 100µg ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10
Images
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Western blot - Human TUBB3 (beta III Tubulin) knockout HCT116 cell lysate (ab257070)
Lane 1: Wild-type HCT116 cell lysate (20µg)
Lane 2: TUBB3 knockout HCT116 cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab52623 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.
ab52623 Anti-beta III Tubulin antibody [EP1569Y] was shown to specifically react with beta III Tubulin in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266900 (knockout cell lysate ab257070) was used. Wild-type and beta III Tubulin knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab52623 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human TUBB3 (beta III Tubulin) knockout HCT116 cell lysate (ab257070)Lane 1: Wild-type HCT116 cell lysate (20µg)
Lane 2: TUBB3 knockout HCT116 cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab215037 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.
ab215037 Anti-beta III Tubulin antibody [EPR19591] was shown to specifically react with beta III Tubulin in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266900 (knockout cell lysate ab257070) was used. Wild-type and beta III Tubulin knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab215037 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human TUBB3 knockout HCT116 cell lysate (ab257070)Homozygous: 1 bp insertion in exon4