Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP690Y] to beta Catenin - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta Catenin antibody [EP690Y] - BSA and Azide free
    See all beta Catenin primary antibodies

  • Description

    Rabbit monoclonal [EP690Y] to beta Catenin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A431, NIH/3T3 and C6 cell lysate. ICC/IF: Wild-type HAP1 cells. Flow Cyt: A431 cells.

  • General notes

    Ab247372 is the carrier-free version of ab68183. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247372 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Western blot - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    All lanes : Anti-beta Catenin antibody [EP690Y] (ab68183) at 1/1000 dilution (Purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 75,90 kDa
    why is the actual band size different from the predicted?



    full-length beta catenin: 90kDa ; C-terminal cleavage fragment: 75kDa (PMID: 15492240).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68183).

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Immunocytochemistry/ Immunofluorescence analysis of parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) cells labeling beta Catenin with Purified ab68183 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68183).
  • Western blot - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Western blot - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Anti-beta Catenin antibody [EP690Y] (ab68183) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 75,90 kDa why is the actual band size different from the predicted?



    full-length beta catenin: 90kDa ; C-terminal cleavage fragment: 75kDa (PMID: 15492240).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68183).

  • Flow Cytometry - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Flow Cytometry - Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling beta Catenin with Purified ab68183 at 1/20 dilution (5 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68183).
  • Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)
    Anti-beta Catenin antibody [EP690Y] - BSA and Azide free (ab247372)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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