Human Desmoglein 3 ELISA Kit (PVA) (ab275902)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Desmoglein 3 ELISA Kit (PVA)
See all Desmoglein 3/PVA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4% Inter-assay Sample n Mean SD CV% Serum 3 7% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
39.06 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 81 79% - 84% Serum 83 80% - 86% Hep Plasma 92 84% - 97% EDTA Plasma 98 95% - 104% Cit plasma 89 87% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Desmoglein 3 ELISA kit (ab275902) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Desmoglein 3 protein in human cell extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Desmoglein 3 with 8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Desmoglein 3 (DSG3, PVA) is a key component of cell-cell junctions in many epithelial type cells. Auto-antibody formation to DSG3 and subsequent loss of adhesive function is the main cause of pemphigus vulgaris, a skin blistering disease. Antibodies for this kit were raised against an immunogen which represents the N-terminal half of the mature protein. This protein does not share high sequence identity in mouse or rat samples (72 and 68%, respectively) and, therefore, this kit is not expected to cross-react.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-2RB (CD122) Capture Antibody 1 x 600ml 10X Human IL-2RB (CD122) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human IL-2RB (CD122) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
Epidermis, tongue, tonsil, esophagus and carcinomas. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Alternative names
- 130 kD pemphigus vulgaris antigen
- 130 kDa pemphigus vulgaris antigen
- Balding
see all -
Database links
- Entrez Gene: 1830 Human
- Omim: 169615 Human
- SwissProt: P32926 Human
- Unigene: 1925 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Desmoglein 3 standard curve in 1X Cell Extraction Buffer PTR.The Desmoglein 3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Desmoglein 3 in human HACAT cell extract and A431 cell extract based on 25 µg/mL and 15 µg/mL extract load respectively.The concentrations of Desmoglein 3 were measured in duplicate and interpolated from the Desmoglein 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Desmoglein 3 concentration was determined to be 2331.46 pg/mL in HACAT cell extract and 1827.74 pg/mL in A431 cell extract.
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Sandwich ELISA - Human Desmoglein 3 ELISA Kit (PVA) (ab275902)To learn more about the advantages of recombinant antibodies see here.
