Human OB Cadherin ELISA Kit (ab267594)
Key features and details
- Sensitivity: 0.8 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human OB Cadherin ELISA Kit
See all OB Cadherin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.8 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93.61 87% - 100% Plasma 87 79% - 91% Cell culture media 114 102% - 128% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human OB Cadherin ELISA Kit is designed for the quantitative determination of OB Cadherin in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human OB Cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and OB Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human OB Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of OB Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects human OB Cadherin, no cross-reativity with Cadherin-4, 6, 8, 12, 13, 17, E-Cadherin, N-Cadherin, P-Cadherin, or VE-Cadherin is observed.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human OB Cadherin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human OB Cadherin Detection Antibody 2 vials Human OB Cadherin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts. -
Involvement in disease
Note=A chromosomal aberration involving CDH11 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with USP6. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CAD11
- CAD11_HUMAN
- Cadherin 11
see all -
Database links
- Entrez Gene: 1009 Human
- Omim: 600023 Human
- SwissProt: P55287 Human
- Unigene: 116471 Human
Images
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Standard curve
Human OB Cadherin ELISA kit (ab267594) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.