Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IgG4 antibody [RM120] (ab238320)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM120] to IgG4
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-IgG4 antibody [RM120]
    See all IgG4 primary antibodies

  • Description

    Rabbit monoclonal [RM120] to IgG4

  • Host species

    Rabbit

  • Specificity

    ab238320 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human IgG4 (Hinge).
    Database link: P01861

  • Positive control

    • WB: Reduced and non-reduced human IgG4. IHC-P: Human lymphoid tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Protein A affinity purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM120

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-IgG4 antibody [RM120] (ab238320)
    Western blot - Anti-IgG4 antibody [RM120] (ab238320)
    All lanes : Anti-IgG4 antibody [RM120] (ab238320) at 2 µg/ml

    Lane 1 : non-reduced (-) and reduced (+) forms of Human IgG1
    Lane 2 : non-reduced (-) and reduced (+) forms of Human IgG2
    Lane 3 : non-reduced (-) and reduced (+) forms of Human IgG3
    Lane 4 : non-reduced (-) and reduced (+) forms of Human IgG4
    Lane 5 : non-reduced (-) and reduced (+) forms of Mouse IgG
    Lane 6 : non-reduced (-) and reduced (+) forms of rat IgG
    Lane 7 : non-reduced (-) and reduced (+) forms of goat IgG

    Predicted band size: 36 kDa



    The Western blot shows ab238320 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [RM120] (ab238320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [RM120] (ab238320)

    Formalin-fixed, Paraffin-embedded human lymphoid tissue stained for Human IgG4 using ab238320 at 2 μg/mL in immunohistochemical analysis.

  • ELISA - Anti-IgG4 antibody [RM120] (ab238320)
    ELISA - Anti-IgG4 antibody [RM120] (ab238320)

    Sandwich ELISA using ab238320 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.

  • ELISA - Anti-IgG4 antibody [RM120] (ab238320)
    ELISA - Anti-IgG4 antibody [RM120] (ab238320)

    A titer ELISA using ab238320. The plate was coated with different amounts of human IgG4. A serial dilution of ab238320 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA - Anti-IgG4 antibody [RM120] (ab238320)
    ELISA - Anti-IgG4 antibody [RM120] (ab238320)

    Detection of human IgG4 in monkey serum. Sandwich ELISA using ab238320 as the capture antibody, and a biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by a HRP conjugated streptavidin.

  • ELISA - Anti-IgG4 antibody [RM120] (ab238320)
    ELISA - Anti-IgG4 antibody [RM120] (ab238320)

    ELISA showing ab238320 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of ab238320 and a monkey IgG binding antibody (positive control) was used as the detection antibody.

  • Anti-IgG4 antibody [RM120] (ab238320)
    Anti-IgG4 antibody [RM120] (ab238320)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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