Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µg 281 433 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM120] to IgG4
Suitable for: WB, IHC-P, ELISA
Reacts with: Human, Recombinant fragment

Product name

Anti-IgG4 antibody [RM120]
See all IgG4 primary antibodies
Description

Rabbit monoclonal [RM120] to IgG4
Host species

Rabbit
Specificity

ab238320 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
IHC-P	Human
WB	Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human IgG4 (Hinge).
Database link: P01861
Positive control
- WB: Reduced and non-reduced human IgG4. IHC-P: Human lymphoid tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

Protein A affinity purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM120
Isotype

IgG
Research areas

Western blot - Anti-IgG4 antibody [RM120] (ab238320)

All lanes : Anti-IgG4 antibody [RM120] (ab238320) at 2 µg/ml

Lane 1 : non-reduced (-) and reduced (+) forms of Human IgG1
Lane 2 : non-reduced (-) and reduced (+) forms of Human IgG2
Lane 3 : non-reduced (-) and reduced (+) forms of Human IgG3
Lane 4 : non-reduced (-) and reduced (+) forms of Human IgG4
Lane 5 : non-reduced (-) and reduced (+) forms of Mouse IgG
Lane 6 : non-reduced (-) and reduced (+) forms of rat IgG
Lane 7 : non-reduced (-) and reduced (+) forms of goat IgG

Predicted band size: 36 kDa

The Western blot shows ab238320 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [RM120] (ab238320)

Formalin-fixed, Paraffin-embedded human lymphoid tissue stained for Human IgG4 using ab238320 at 2 μg/mL in immunohistochemical analysis.
ELISA - Anti-IgG4 antibody [RM120] (ab238320)

Sandwich ELISA using ab238320 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-IgG4 antibody [RM120] (ab238320)

A titer ELISA using ab238320. The plate was coated with different amounts of human IgG4. A serial dilution of ab238320 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-IgG4 antibody [RM120] (ab238320)

Detection of human IgG4 in monkey serum. Sandwich ELISA using ab238320 as the capture antibody, and a biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by a HRP conjugated streptavidin.
ELISA - Anti-IgG4 antibody [RM120] (ab238320)

ELISA showing ab238320 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of ab238320 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Anti-IgG4 antibody [RM120] (ab238320)

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