Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR707(N)] to beta 2 Adrenergic Receptor
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]
See all beta 2 Adrenergic Receptor primary antibodies
Description

Rabbit monoclonal [EPR707(N)] to beta 2 Adrenergic Receptor
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver, Human skeletal muscle, Mouse heart, Rat heart, Mouse kidney, Rat kidney, and A431 lysates; IHC-P: Rat stomach, Human stomach and Mouse cerebrum tissues; IHC-Fr: Mouse stomach tissue section.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR707(N)
Isotype

IgG
Research areas

Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

All lanes : Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136) at 0.1 µg/ml (purified)

Lane 1 : A431 ( Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 2 : Human skeletal muscle lysates
Lane 3 : Human fetal liver lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat heart lysates
Lane 6 : Mouse kidney lysates
Lane 7 : Rat kidney lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 46 kDa

Blocking and diluting buffer: 5% NFDM/TBST

The 50-90kda bands detected by ab182136 are consistent with many literatures, like PMID: 2836733, PMID: 17570158, PMID: 11701618, PMID: 2545714, PMID: 16708760.
Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

IHC image of beta 2 Adrenergic Receptor staining in a section of frozen human prostate carcinoma performed on a Leica BOND^TM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab182136, 1/750 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1/100 (1.3 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1:100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136)

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