Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bag3 antibody [EPR20207-4-3] (ab246224)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20207-4-3] to Bag3
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Bag3 antibody [EPR20207-4-3]
    See all Bag3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20207-4-3] to Bag3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle lysate; HeLa and HL-60 whole cell lysates; Mouse and rat heart lysates. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Bag3 antibody [EPR20207-4-3] (ab246224)
    Western blot - Anti-Bag3 antibody [EPR20207-4-3] (ab246224)
    All lanes : Anti-Bag3 antibody [EPR20207-4-3] (ab246224) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat heart lysate
    Lane 5 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 8 seconds; Lane 2: 70 seconds; Lane 3: 10 seconds; Lane 4: 8 seconds; Lane 5: 180 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17974966).

    Negative control: HL-60 (PMID: 17974966).

  • Flow Cytometry - Anti-Bag3 antibody [EPR20207-4-3] (ab246224)
    Flow Cytometry - Anti-Bag3 antibody [EPR20207-4-3] (ab246224)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HL-60 (human promyelocytic leukemia cell line; left panel) and HeLa (human epithelial cell line from cervix adenocarcinoma; right panel) cell lines labeling Bag3 with ab246224 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HL-60 (PMID: 17974966).

  • Anti-Bag3 antibody [EPR20207-4-3] (ab246224)
    Anti-Bag3 antibody [EPR20207-4-3] (ab246224)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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