Anti-ATXN2L antibody (ab184834)
Key features and details
- Rabbit polyclonal to ATXN2L
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATXN2L antibody
See all ATXN2L primary antibodies -
Description
Rabbit polyclonal to ATXN2L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human ATXN2L aa 450-550.
Database link: Q8WWM7 -
Positive control
- IHC-P: Human testis, kidney, tonsil and duodenum tissue; ICC: A431 whole cells; WB: U-2 OS whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATXN2L antibody (ab184834)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ATXN2L in the cytoplasm of germinal center cells with ab184834 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-ATXN2L antibody (ab184834)Immunofluorescent analysis of PFA/Triton X-100-treated A431 (human epidermoid carcinoma cell line) whole cells labeling ATXN2L in the cytosol with ab184834 at 2 µg/ml.
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Western blot - Anti-ATXN2L antibody (ab184834)All lanes : Anti-ATXN2L antibody (ab184834) at 0.4 µg/ml
Lane 1 : Target specific siRNA probe #1 transfected into U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 2 : Target specific siRNA probe #2 transfected into U-2 OS whole cell lysate
Lane 3 : Control siRNA transfected into U-2 OS whole cell lysate
Predicted band size: 113 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATXN2L antibody (ab184834)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ATXN2L in the cytoplasm of glomeruli cells with ab184834 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATXN2L antibody (ab184834)Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling ATXN2L in the cytoplasm of glandular cells with ab184834 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATXN2L antibody (ab184834)Immunohistochemical analysis of paraffin-embedded human testis tissue labeling ATXN2L in the cytoplasm of Leydig cells with ab184834 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
