Anti-B MyB antibody (ab12296)
Key features and details
- Rabbit polyclonal to B MyB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-B MyB antibody
See all B MyB primary antibodies -
Description
Rabbit polyclonal to B MyB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human B MyB aa 500-600 (internal sequence).
(Peptide available asab12310) -
Positive control
- Purchase matching WB positive control:Recombinant Human B MyB protein
- This antibody gave a positive signal in HEK293 Whole Cell Lysate Transiently Overexpressing B-Myb
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseXenopus laevisApplication Abreviews Notes WB Notes Application notesWB: 1/2500. Detects a band of approximately ~90 kDa (predicted molecular weight: 79 kDa).
ab12296 was tested in ICC/IF on HeLa cells (1/100, 1/200, 1/500) and gave high background staining. It appears the antibody may not work in this application.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene. -
Sequence similarities
Contains 3 HTH myb-type DNA-binding domains. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4605 Human
- Entrez Gene: 17865 Mouse
- Omim: 601415 Human
- SwissProt: P10244 Human
- SwissProt: P48972 Mouse
- SwissProt: P52551 Xenopus laevis
- Unigene: 179718 Human
- Unigene: 4594 Mouse
see all -
Alternative names
- B-Myb antibody
- BMyB antibody
- MGC15600 antibody
see all
Images
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Western blot - Anti-B MyB antibody (ab12296)Michael Korenja and Alexander Brehm
A: 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.
B: negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.
A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.
ab12296 dilution: 200
µ g/ml.
Exposure was for 10 minutes.
A: 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.
B: negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.
A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.
ab12296 dilution: 200 µg/ml. Exposure was for 10 minutes. -
Western blot - Anti-B MyB antibody (ab12296)Anti-B MyB antibody (ab12296) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing B-Myb at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Datasheets and documents
References (7)
ab12296 has been referenced in 7 publications.
- Li X et al. miR-30a inhibits androgen-independent growth of prostate cancer via targeting MYBL2, FOXD1, and SOX4. Prostate 80:674-686 (2020). PubMed: 32294305
- Porta S et al. Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases. J Neuropathol Exp Neurol 74:380-7 (2015). PubMed: 25756586
- Heinrichs S et al. MYBL2 is a sub-haploinsufficient tumor suppressor gene in myeloid malignancy. Elife 2:e00825 (2013). WB ; Human . PubMed: 23878725
- Chandra S et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci 126:3782-95 (2013). PubMed: 23750012
- Druz A et al. Large-scale screening identifies a novel microRNA, miR-15a-3p, which induces apoptosis in human cancer cell lines. RNA Biol 10:287-300 (2013). WB ; Human . PubMed: 23353574
- Zhan M et al. The B-MYB transcriptional network guides cell cycle progression and fate decisions to sustain self-renewal and the identity of pluripotent stem cells. PLoS One 7:e42350 (2012). ChIP . PubMed: 22936984
- Tarasov KV et al. B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells. PLoS ONE 3:e2478 (2008). WB ; Human, Mouse . PubMed: 18575582
Images
-
Western blot - Anti-B MyB antibody (ab12296) Michael Korenja and Alexander Brehm
A: 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.
B: negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.
A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.
ab12296 dilution: 200
µ g/ml.
Exposure was for 10 minutes.
A: 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.
B: negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.
A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.
ab12296 dilution: 200 µg/ml. Exposure was for 10 minutes. -
Western blot - Anti-B MyB antibody (ab12296)Anti-B MyB antibody (ab12296) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing B-Myb at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
