Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TXNDC9 antibody [EPR15238] (ab185959)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15238] to TXNDC9
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-TXNDC9 antibody [EPR15238]
See all TXNDC9 primary antibodies
Description

Rabbit monoclonal [EPR15238] to TXNDC9
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, K562, Jurkat, Raji, SW460, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates. IHC-P: Human colon and astrocytoma tissues.
General notes
Previously labelled as Thioredoxin domain containing 9.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15238
Isotype

IgG
Research areas

Western blot - Anti-TXNDC9 antibody [EPR15238] (ab185959)

All lanes : Anti-TXNDC9 antibody [EPR15238] (ab185959) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TXNDC9 knockout HeLa cell lysate
Lane 3 : K-562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Lanes 1-3: Merged signal (red and green). Green - ab185959 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.

ab185959 Anti-TXNDC9 antibody [EPR15238] was shown to specifically react with TXNDC9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265420 (knockout cell lysate ab258257) was used. Wild-type and TXNDC9 knockout samples were subjected to SDS-PAGE. ab185959 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15238] (ab185959)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TXNDC9 with ab185959 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15238] (ab185959)

Immunohistochemical analysis of paraffin-embedded Human astocytoma tissue labeling TXNDC9 with ab185959 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-TXNDC9 antibody [EPR15238] (ab185959)

All lanes : Anti-TXNDC9 antibody [EPR15238] (ab185959) at 1/10000 dilution

Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raji cell lysate
Lane 4 : SW480 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Immunoprecipitation - Anti-TXNDC9 antibody [EPR15238] (ab185959)

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab185959 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Western blot - Anti-TXNDC9 antibody [EPR15238] (ab185959)

All lanes : Anti-TXNDC9 antibody [EPR15238] (ab185959) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Anti-TXNDC9 antibody [EPR15238] (ab185959)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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