Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TXNDC9 antibody [EPR15239] (ab191405)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15239] to TXNDC9
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-TXNDC9 antibody [EPR15239]
See all TXNDC9 primary antibodies
Description

Rabbit monoclonal [EPR15239] to TXNDC9
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, K562, Jurkat, Raji, SW480, C6, Raw264.7 and NIH/3T3 cell lysates. IHC-P: Human squamous cell carcinoma of cervix tissue. ICC/IF: SW480 cells.
General notes
Previously labelled as Thioredoxin domain containing 9.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15239
Isotype

IgG
Research areas

Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TXNDC9 knockout HeLa cell lysate
Lane 3 : K-562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Lanes 1-3: Merged signal (red and green). Green - ab191405 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.

ab191405 Anti-TXNDC9 antibody [EPR15239] was shown to specifically react with TXNDC9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265420 (knockout cell lysate ab258257) was used. Wild-type and TXNDC9 knockout samples were subjected to SDS-PAGE. ab191405 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15239] (ab191405)

Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling TXNDC9 with ab191405 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TXNDC9 antibody [EPR15239] (ab191405)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized SW480 cells labeling TXNDC9 with ab191405 at 1/400 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

The two negative controls used anti-TXNDC9 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/10000 dilution + K562 cell lysate at 20 µg

Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : SW80 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TXNDC9 antibody [EPR15239] (ab191405)

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