Anti-TXNDC9 antibody [EPR15239] (ab191405)
![Anti-TXNDC9 antibody [EPR15239] (ab191405)](https://www.abcam.com/ps/products/191/ab191405/Images/ab191405-393572-anti-txndc9-antibody-epr15239-western-blot-human-knockout-lysate.jpg "Anti-TXNDC9 antibody [EPR15239] (ab191405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15239] to TXNDC9
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TXNDC9 antibody [EPR15239]
See all TXNDC9 primary antibodies -
Description
Rabbit monoclonal [EPR15239] to TXNDC9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, K562, Jurkat, Raji, SW480, C6, Raw264.7 and NIH/3T3 cell lysates. IHC-P: Human squamous cell carcinoma of cervix tissue. ICC/IF: SW480 cells.
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General notes
Previously labelled as Thioredoxin domain containing 9.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15239 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TXNDC9 knockout HeLa cell lysate
Lane 3 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaLanes 1-3: Merged signal (red and green). Green - ab191405 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.
ab191405 Anti-TXNDC9 antibody [EPR15239] was shown to specifically react with TXNDC9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265420 (knockout cell lysate ab258257) was used. Wild-type and TXNDC9 knockout samples were subjected to SDS-PAGE. ab191405 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15239] (ab191405)](https://www.abcam.com/ps/products/191/ab191405/Images/ab191405-228736-ab191405IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15239] (ab191405)Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling TXNDC9 with ab191405 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TXNDC9 antibody [EPR15239] (ab191405)](https://www.abcam.com/ps/products/191/ab191405/Images/ab191405-228735-ab191405ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TXNDC9 antibody [EPR15239] (ab191405)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized SW480 cells labeling TXNDC9 with ab191405 at 1/400 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).
The two negative controls used anti-TXNDC9 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
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![Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)](https://www.abcam.com/ps/products/191/ab191405/Images/ab191405-228739-ab191405WB1.jpg)
Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/10000 dilution + K562 cell lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)](https://www.abcam.com/ps/products/191/ab191405/Images/ab191405-228738-ab191405WB2.jpg)
Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : SW80 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)](https://www.abcam.com/ps/products/191/ab191405/Images/ab191405-228737-ab191405WB3.jpg)
Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-TXNDC9 antibody [EPR15239] (ab191405)](https://www.abcam.com/ps/products/191/ab191405/Images/ab191405-7-benefits-of-recombinant-antibodies.png)
Anti-TXNDC9 antibody [EPR15239] (ab191405)
