Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2204Y] to B MyB (phospho T487) - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free
    See all B MyB primary antibodies

  • Description

    Rabbit monoclonal [EPR2204Y] to B MyB (phospho T487) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab232521 detects B MyB phosphorlyated on threonine 487.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human urinary bladder carcinoma tissue.

  • General notes

    ab232521 is the carrier-free version of ab76009. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232521 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    ab76009, at 1/100 dilution, staining B MyB in human urinary bladder carcinoma by immunohistochemistry using paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76009).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)
    Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)
    ab76009, at 1/100 dilution, staining B MyB in HeLa cells by immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76009).

  • Flow Cytometry - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)
    Flow Cytometry - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Overlay histogram showing HeLa cells stained with ab76009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76009, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76009).

  • Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)
    Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

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