Anti-ATP8 antibody [1G3-1H11-1C3] (ab243667)
![Anti-ATP8 antibody [1G3-1H11-1C3] (ab243667)](https://www.abcam.com/ps/products/243/ab243667/Images/ab243667-333760-anti-atp8-antibody-1g3-1h11-1c3-western-blot.jpg "Anti-ATP8 antibody [1G3-1H11-1C3] (ab243667)")
Key features and details
- Mouse monoclonal [1G3-1H11-1C3] to ATP8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-ATP8 antibody [1G3-1H11-1C3] -
Description
Mouse monoclonal [1G3-1H11-1C3] to ATP8 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATP8 (N terminal).
Database link: P03928 -
Positive control
- WB: Mitochondria from cultured normal Control Human Dermal Fibroblasts Neonatal (HDFn).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, HEPES -
Concentration information loading... -
Purity
Protein L purified -
Purification notes
Protein L affinity chromatography from Fetal Bovine Serum-containing medium. Purity: Near homogeneity as assessed by SDS-PAGE and Coomasie Blue staining. -
Clonality
Monoclonal -
Clone number
1G3-1H11-1C3 -
Isotype
IgG2b -
Light chain type
kappa
Images
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Western blot - Anti-ATP8 antibody [1G3-1H11-1C3] (ab243667)All lanes : Anti-ATP8 antibody [1G3-1H11-1C3] (ab243667) at 2 µg/ml
Lane 1 : Mitochondria from cultured normal Control Human Dermal Fibroblasts Neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of Ethidium Bromide
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Predicted band size: 8 kDaMitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and transferred to a PVDF membrane in CAPS buffer.
Right panel shows total protein.