Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13908] to ATP5G1/G2/G3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATP5G1/G2/G3 antibody [EPR13908]
    See all ATP5G1/G2/G3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13908] to ATP5G1/G2/G3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP5G1/G2/G3 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P05496

  • Positive control

    • HeLa and Human fetal heart lysates; Human liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified)

    Lane 1 : mouse heart lysate
    Lane 2 : rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 8 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified) + human fetal heart lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 8 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab180149 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    Immuohistochemical analysis of Paraffin-embedded Human liver tissue labeling ATP5G1 with unpurified ab180149 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

  • Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    Western blot analysis of fetal heart lysate immunoprecipitated with unpurified ab180149 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

  • Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
    Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.