Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13908] to ATP5G1/G2/G3
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-ATP5G1/G2/G3 antibody [EPR13908]
See all ATP5G1/G2/G3 primary antibodies
Description

Rabbit monoclonal [EPR13908] to ATP5G1/G2/G3
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP5G1/G2/G3 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P05496
Positive control
- HeLa and Human fetal heart lysates; Human liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13908
Isotype

IgG
Research areas

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified)

Lane 1 : mouse heart lysate
Lane 2 : rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 8 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified) + human fetal heart lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 8 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab180149 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : Human fetal heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

Immuohistochemical analysis of Paraffin-embedded Human liver tissue labeling ATP5G1 with unpurified ab180149 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.
Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

Western blot analysis of fetal heart lysate immunoprecipitated with unpurified ab180149 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

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