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Signal Transduction Metabolism Energy Metabolism

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free
    See all ATP citrate lyase primary antibodies
  • Description

    Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247346 is the carrier-free version of ab53007. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247346 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP737Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Images

  • Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
    Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
    All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 15 minutes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa
    Observed band size: 122 kDa



    This data was developed using ab53007, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
    Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
    All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : C6 (Rat glial tumor glial cell) treated with 100ng Calyculin A for 60 minutes whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa
    Observed band size: 122 kDa



    This data was developed using ab53007, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
    Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
    All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (unpurified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with insulin whole cell lysate.
    Lane 2 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

    Predicted band size: 122 kDa


    Exposure time: 3 minutes


    This data was developed using ab53007, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)
    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free (ab247346)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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