Anti-ASF1b antibody [6G7G4] (ab233559)
Key features and details
- Mouse monoclonal [6G7G4] to ASF1b
- Suitable for: WB, Flow Cyt
- Reacts with: Human, African green monkey, Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-ASF1b antibody [6G7G4]
See all ASF1b primary antibodies -
Description
Mouse monoclonal [6G7G4] to ASF1b -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, African green monkey, Chinese hamster
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant full length protein corresponding to Human ASF1b aa 1 to the C-terminus. Expressed in E. coli.
Database link: Q9NVP2 -
Positive control
- WB: Recombinant human ASF1b protein (amino acids 1-202). ASF1b (amino acids 1-202) - hIgGFc transfected HEK-293 cell lysate. HeLa, COS-7, HCT 116 and CHO3D10 cell lysate. Flow Cytometry: K562 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
6G7G4 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells, labeling ASF1b with ab233559 at a 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-ASF1b antibody [6G7G4] (ab233559) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 3 : HCT 116 (Human colorectal carcinoma cell line) cell lysate
Lane 4 : CHO3D10 cell lysate
Predicted band size: 22.4 kDa
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All lanes : Anti-ASF1b antibody [6G7G4] (ab233559) at 1/500 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ASF1b (amino acids 1-202) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 22.4 kDa
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Anti-ASF1b antibody [6G7G4] (ab233559) at 1/500 dilution + Recombinant human ASF1b protein (amino acids 1-202)
Predicted band size: 22.4 kDa(Expected MW is 48.4 kDa)