Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free
    See all AS160 primary antibodies

  • Description

    Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects AS160 when phosphorylated at Threonine 642.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T cell lysate (insulin-treated)

  • General notes

    ab218844 is the carrier-free version of ab131214 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab218844 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1.12 µg/ml

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate
    Lane 2 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate
    Lane 3 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 146 kDa



    This data was developed using ab131214, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    This data was developed using ab131214, the same antibody clone in a different buffer formulation.Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with ab131214 at a dilution of 1:1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.
  • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilution

    Lane 1 : 293T cell lysate - untreated
    Lane 2 : 293T cell lysate - insulin-treated

    Predicted band size: 146 kDa



    This data was developed using ab131214, the same antibody clone in a different buffer formulation.

    Bottom pannel shows detection of total AS160 with a general anti AS160 antibody.

  • OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    This data was developed using ab131214, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)
    Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)

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