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Anti-GNE antibody [EPR15059] - C-terminal (ab189927)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15059] to GNE - C-terminal
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GNE antibody [EPR15059] - C-terminal
    See all GNE primary antibodies

  • Description

    Rabbit monoclonal [EPR15059] to GNE - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, fetal kidney, fetal liver, HeLa, A549 and K562 lysates; HeLa cells.

  • General notes

     This product was previously labelled as GLCNE

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15059

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)
    Western blot - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)
    All lanes : Anti-GNE antibody [EPR15059] - C-terminal (ab189927) at 1/1000 dilution

    Lane 1 : Human fetal liver
    Lane 2 : Human fetal kidney
    Lane 3 : Human fetal brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)
    Western blot - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)
    All lanes : Anti-GNE antibody [EPR15059] - C-terminal (ab189927) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)
    Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling GNE with ab189927 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution.  Counter stained with DAPI.

  • Immunoprecipitation - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)
    Immunoprecipitation - Anti-GNE antibody [EPR15059] - C-terminal (ab189927)

    Western blot analysis of GNE in A549 cell lysate immunoprecipitated using ab189927 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-GNE antibody [EPR15059] - C-terminal (ab189927)
    Anti-GNE antibody [EPR15059] - C-terminal (ab189927)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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